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Beta-Galactosidase activity assay with CPRG as substrate


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3 replies to this topic

#1 anahi

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Posted 16 April 2009 - 01:35 PM

Hi all,
I'm looking for a good protocol for Beta-Galactosidase Activity Assays that can be adapted to a 96-well plate format. Also, I need to use CPRG as the substrate, I bought it from Roche and don't know how to prepare it (in which solution and concentration?).
Thanks for the help!!!
Anahi

#2 mdfenko

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Posted 17 April 2009 - 08:30 AM

you can adapt this assay to a 96 well plate:

beta galactosidase assay (worthington)
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#3 anahi

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Posted 17 April 2009 - 09:49 AM

[quote name='mdfenko' date='Apr 17 2009, 12:30 PM' post='22102']
you can adapt this assay to a 96 well plate:

beta galactosidase assay (worthington)
[/quot

Unfortunately, that protocol won't be useful, it doesn't guide me on how to prepare the substrate CPRG but also I'm doing this liquid Beta Gal assay to assess LacZ activation in a Y2H strain.
Thanks anyway,

#4 genehunter

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Posted 17 April 2009 - 04:23 PM

anahi,

check this out: Groth et al (1998) Anal Biochem 258: 141-3

http://www.sciencedi...dd59664eb5334e2




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