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Please anybody can explain the cloning related to plant vector construction


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5 replies to this topic

#1 KAMALAKER

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Posted 16 April 2009 - 03:11 AM

In Cloning what is the use of polylinker usually they wil use pUC plasmids what is this ?

Can anybody explain

#2 phage434

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Posted 16 April 2009 - 04:10 AM

A polylink is a DNA sequence that contains many of the common restriction sites. Inserting it into a vector allows you to clone virtually any restriction cut piece of DNA into that vector.

#3 KAMALAKER

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Posted 16 April 2009 - 07:20 AM

thanking u very much

can u give one example I am new to this area

#4 phage434

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Posted 16 April 2009 - 08:06 PM

Examine the sequene of the cloning site of a common cloning vector such as pUC19. When these were first developed (1980s I think) there were papers published on them, which Pubmed could probably find, for example:

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879

#5 KAMALAKER

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Posted 17 April 2009 - 06:12 AM

Examine the sequene of the cloning site of a common cloning vector such as pUC19. When these were first developed (1980s I think) there were papers published on them, which Pubmed could probably find, for example:

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879




thanking u very much sir

#6 KAMALAKER

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Posted 17 April 2009 - 06:35 AM

what are all the basic things need to know before starting the cloning work?




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