5 replies to this topic

[KAMALAKER]

In Cloning what is the use of polylinker  usually they wil use pUC plasmids what is this ?

Can anybody explain

[phage434]

A polylink is a DNA sequence that contains many of the common restriction sites.  Inserting it into a vector allows you to clone virtually any restriction cut piece of DNA into that vector.

[KAMALAKER]

thanking u very much  

can u give one example  I am new to this area

[phage434]

Examine the sequene of the cloning site of a common cloning vector such as pUC19.   When these were first developed (1980s I think) there were papers published on them, which Pubmed could probably find, for example:

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879

[KAMALAKER]

phage434, on Apr 17 2009, 09:36 AM, said:

Examine the sequene of the cloning site of a common cloning vector such as pUC19.   When these were first developed (1980s I think) there were papers published on them, which Pubmed could probably find, for example:

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J
Gene. 1982 Oct;19(3):259-68.
PMID: 6295879

thanking u very  much sir

[KAMALAKER]

what are all the basic things need to know before starting the cloning work?