I want to extract genomic DNA which is up to several mega base pair from Burkholderia, are there any commercial kits capble to do so? Thanks
Extraction of large size genomic DNA
Started by Nrelo, Apr 16 2009 01:12 AM
3 replies to this topic
#1
Posted 16 April 2009 - 01:12 AM
#2
Posted 16 April 2009 - 04:08 AM
What do you want to do with this DNA. If you need it in whole chromosomes, then you need to embed cells in agarose and digest them in-place. If you just need DNA for sequencing, library construction, or PCR, then any normal genomic prep will work fine. I've had a lot of success with the Zymed 96 well plate format preps, but also with the Sambrook CTAB protocol.
#3
Posted 16 April 2009 - 04:54 AM
phage434, on Apr 16 2009, 04:08 AM, said:
What do you want to do with this DNA. If you need it in whole chromosomes, then you need to embed cells in agarose and digest them in-place. If you just need DNA for sequencing, library construction, or PCR, then any normal genomic prep will work fine. I've had a lot of success with the Zymed 96 well plate format preps, but also with the Sambrook CTAB protocol.
I have inserted a gene into the bacterial genome and want to find out the insertion site. So I need to extract the genomic DNA, digest it with a R.E, ligate the fragments and sequence them. In this case, any normal genomic prep will work?
#4
Posted 16 April 2009 - 08:00 PM
Yes.














