3 replies to this topic

[Nrelo]

I want to extract genomic DNA which is up to several mega base pair from Burkholderia, are there any commercial kits capble to do so? Thanks

[phage434]

What do you want to do with this DNA.   If you need it in whole chromosomes, then you need to embed cells in agarose and digest them in-place.  If you just need DNA for sequencing, library construction, or PCR, then any normal genomic prep will work fine.  I've had a lot of success with the Zymed 96 well plate format preps, but also with the Sambrook CTAB protocol.

[Nrelo]

phage434, on Apr 16 2009, 04:08 AM, said:

What do you want to do with this DNA.   If you need it in whole chromosomes, then you need to embed cells in agarose and digest them in-place.  If you just need DNA for sequencing, library construction, or PCR, then any normal genomic prep will work fine.  I've had a lot of success with the Zymed 96 well plate format preps, but also with the Sambrook CTAB protocol.

I have inserted a gene into the bacterial genome and want to find out the insertion site. So I need to extract the genomic DNA, digest it with a R.E, ligate the fragments and sequence them. In this case, any normal genomic prep will work?

[phage434]

Yes.