Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

How to synchronize HEK293

  • Please log in to reply
1 reply to this topic

#1 R.Lolo



  • Members
  • Pip
  • 3 posts

Posted 14 April 2009 - 12:56 AM

Dear All,

i'm trying to investigate subcellular localisation of certain proteins during cell cycle - it is just a pilot experiment - I would like to synchronise cells and then take pictures in time steps. At that step I don't care about having cells in a certain phase - I just want to take a look if there are any cganges during the cycle.
Does anyone know a simple method to synchronize HEK293? I don't want to buy new reagents- I was thinking about serum starvation or mitotic shake off. Does anyone have a good protocol, or a good tip how to deal with it? Let me add that I need to tranfect my cells with plasmid - when should I do it - before or after synchronisation?
I would be more than grateful.
Rafal L

Edited by R.Lolo, 14 April 2009 - 01:25 AM.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,507 posts

Posted 14 April 2009 - 04:51 PM

serum starvation is the easiest: Just add serum-free medium (DMEM will be fine) for 48 hours, and then perform your experiment.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.