Storage of slides
Posted 13 April 2009 - 06:58 PM
Posted 13 April 2009 - 08:27 PM
You can either store your cells after you've fixed them, store in PBS or PBS with BSA and sodium azide for a few days in the fridge (sealed with parafilm to prevent drying out). Or after you have completed the staining. I routinely store my slides in the fridge, in a slide box or wrapped in foil to prevent fading.
I'm not sure how far apart your time points are, so you should choose which is the best time schedule for you.
hope that helps
Posted 14 April 2009 - 12:55 AM
HI, I usually store the cell in 1% bsa in 4oc for up to a week, time concerning immunobloting with primary antibody should optimized empirically range from 1 hour to overnight. I think rodamine glows better than fitc.