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Mung Bean Nuclease problem


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#1 Chris C

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Posted 08 August 2002 - 03:08 PM

Has anyone used mung bean nuclease and found that instead of just degrading ssDNA overhangs from linear dsDNA, it pretty much degrades it entirely?!

I'm using NEB's MBN, and although I did use more than recommended, for a twice as long as suggested, I didn't predict that it would have this effect!

Can anyone help please?

Thanks,   Chris


#2 sponach1

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Posted 14 October 2002 - 04:33 AM

Hi Chris,
I have been working with MBN.And I had the same problem.

The reason why the enzyme suddenly digests dsDNS is that it gets unspecific when you use a high amount of it (see Maniatis).I recommend testing the proper units as I have been doing.

Chris

#3 tfitzwater

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Posted 24 October 2002 - 10:22 AM

When I had this problem, switching to higher purity water seemed to solve the problem. Possibly due to zinc in the water?




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