B-per GST fusion protein purification kit
Posted 13 April 2009 - 12:56 PM
Posted 07 May 2009 - 08:58 AM
Understanding Life One protein at a time...
The peptide company
Understanding Life One Protein At a Time...
Posted 10 May 2009 - 12:14 AM
It is easy to Purifiy the GST tagged protein , i do agree that the expression levels of GST tagged proteins are very less.
down load the pdf manual from GE from above link and try to see for ur protein
I need to purify GST tagged protein by using B-per reagent from pierce.(I used pGEX2T vector).Is there anyone used this kit? I have used Y-Per reagent before and I have loads of problems. My protein yield was so low. I had also low amount GST band except GST tagged protein on SDS. I donít want to face with these problems again. I will use this protein to immunize rabbit. Any help would be appreciated.