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B-per GST fusion protein purification kit


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#1 bsengez

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Posted 13 April 2009 - 12:56 PM

I need to purify GST tagged protein by using B-per reagent from pierce.(I used pGEX2T vector).Is there anyone used this kit? I have used Y-Per reagent before and I have loads of problems. My protein yield was so low. I had also low amount GST band except GST tagged protein on SDS. I donít want to face with these problems again. I will use this protein to immunize rabbit. Any help would be appreciated.

#2 LifeTein Peptide

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Posted 07 May 2009 - 08:58 AM

GST should be almost the easiest protein for purification. Do not buy p-per kit but just the column and prepare other reagents by yourself. Have your check your GST protein first? If plenty, do dialyze and then purification.


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#3 sudhakar mutyala

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Posted 10 May 2009 - 12:14 AM

Hi

It is easy to Purifiy the GST tagged protein , i do agree that the expression levels of GST tagged proteins are very less.

http://www5.gelifesc...52573D9006F2C34
down load the pdf manual from GE from above link and try to see for ur protein

Regards
Sudhakar

I need to purify GST tagged protein by using B-per reagent from pierce.(I used pGEX2T vector).Is there anyone used this kit? I have used Y-Per reagent before and I have loads of problems. My protein yield was so low. I had also low amount GST band except GST tagged protein on SDS. I donít want to face with these problems again. I will use this protein to immunize rabbit. Any help would be appreciated.






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