3 replies to this topic

[c0ok1e]

Any of you heard of NPB medium before? IM not sure if it is a standard medium or a special medium created by the researcher. I am an amateur in research and would like your help. The composition of the medium is:

10g tryptone peptone
2g yeast extract
1g mgso4.7h2o
1g k2hpo4
0.5g kh2po4
5g glucose

I cannot find the information on the medium online. I would like to know the function of each component of the medium and why they add the component into the medium.

The literature where i encounter this medium is

Tanaka Y, Hanada S, Manome A, Tsuchida T, Kurane R, Nakamura K, Kamagata Y (2004). "Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth". Int. J. Syst. Evol. Microbiol. 54: 955–959

Thanks

[c0ok1e]

Hi again,

I guess I have found the function of most of the components. The remaining components that I am not sure of is MgSO4. I guess MgSO4 provides Mg++ and S to the culture but doesnt the yeast extract already provide the Mg++ ions and peptone provide the S?

Also why is the Mg++ so important that it needs to add extra MgSO4 for it? I think that Mg act as a cofactor for enzymes but does it really need a high amount?

[hanming86]

c0ok1e, on Apr 12 2009, 11:46 PM, said:

Hi again,

I guess I have found the function of most of the components. The remaining components that I am not sure of is MgSO4. I guess MgSO4 provides Mg++ and S to the culture but doesnt the yeast extract already provide the Mg++ ions and peptone provide the S?

Also why is the Mg++ so important that it needs to add extra MgSO4 for it? I think that Mg act as a cofactor for enzymes but does it really need a high amount?

tryptone = amino acid source ( N and C )
glucose = carbon source
yeast = growth factor mainly.
the key is actually Mg . mgcl2 probably would work fine. anyway, Mg2+ serves as co factor for alot of enzyme . even on ribosome and used to stabilize dNTP.

the phosphate is there to help resist the change in pH.

[c0ok1e]

hanming86, on Apr 13 2009, 05:29 AM, said:

c0ok1e, on Apr 12 2009, 11:46 PM, said:

Hi again,

I guess I have found the function of most of the components. The remaining components that I am not sure of is MgSO4. I guess MgSO4 provides Mg++ and S to the culture but doesnt the yeast extract already provide the Mg++ ions and peptone provide the S?

Also why is the Mg++ so important that it needs to add extra MgSO4 for it? I think that Mg act as a cofactor for enzymes but does it really need a high amount?

tryptone = amino acid source ( N and C )
glucose = carbon source
yeast = growth factor mainly.
the key is actually Mg . mgcl2 probably would work fine. anyway, Mg2+ serves as co factor for alot of enzyme . even on ribosome and used to stabilize dNTP.

the phosphate is there to help resist the change in pH.

thats what I found out.. But thanks anyway.. U help to confirm my findings.. I still have a lot more to learn