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primers for mRNA probe synthesis


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#1 plxso1

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Posted 11 April 2009 - 11:47 AM

Hi everyone,
I have to design a probe for in situ hybridisation, but the coding sequence for my gene is very short (~200bp). I've been told I can design a primer for the UTRs to get a bigger product, but I don't know where it starts..
I was wondering if someone could help me? :lol: Should I use a polyT primer to anneal to the polyA tail?
Thank you in advance! :)





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