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Transfection in vivo


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#1 anonymous

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Posted 15 January 2001 - 10:00 PM

Hi, all,

I need to transfect a plasmid into mouse liver and see the expression. Which type of liposome I should use? Is there any commercial product available?

Thanks in advance!


#2 anonymous

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Posted 16 January 2001 - 10:00 PM

Yes there is a commercial product available - TransIT In Vivo Delivery System distributed by Panvera Corp. It works well with great expression levels in the liver.

#3 anonymous

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Posted 18 January 2001 - 10:00 PM

Thank you for your information.

I got the detailed information about the kit. However, there is two doubts. One is the volumn injected (2.2ml for 10ug DNA. Is is too much for a normal mouse, say 20-30g? The other one is the strain of the mouse. Does it work better in some strains than others. How about SCID?

Thank you and looking forward to further information.





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