A sad beginning but a good ending?
Posted 10 April 2009 - 07:46 AM
I think this is something weird.
Lately i been running SDS page, the tracking dye kinda diffuse in the beginning after going through the stacking gel . then in the mid way it sharpened up but still with some diffusion on the front . then in the end everything condensed up to a shart band looking good. but i thought everyhting supposed to condense up after passing the stacking gel.
The resolution of protein seems to be affected from this too.
I m suspecting something wrong with the buffer used to make the resolving gel
What do you guys think?
any hand on experience on this situation?
Posted 13 April 2009 - 07:26 AM
genius does what it must
i do what i get paid to do
Posted 13 April 2009 - 04:35 PM
Posted 21 April 2009 - 02:05 PM
Posted 22 April 2009 - 08:21 AM
Running buffer with a pH of 7.5 = Good game.
Posted 21 June 2009 - 06:45 PM
can i ask what is the normal pH range for running buffer? thanks..