Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...
0
3 replies to this topic
#2
mdfenko
-
- Active Members
-
- 2,251 posts
an elder
79
Excellent
Excellent
Posted 13 April 2009 - 07:25 AM
it depends on the actual mw of the proteins.
1kDa difference is more significant in a small protein than a large one.
1kDa difference is more significant in a small protein than a large one.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
gfischer
-
- Global Moderators
-
- 179 posts
Veteran
7
Neutral
Neutral
Posted 15 April 2009 - 11:37 AM
alessandro, on Apr 10 2009, 09:40 AM, said:
Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...
You may not be able to. Westerns aren't necesarily exact, you usually need to give a band about a 5kDa margin of error.
Above all things, if kindness is your king,
then heaven will be yours, before you meet your end
then heaven will be yours, before you meet your end
#4
mikew
-
- Active Members
-
- 80 posts
Enthusiast
0
Neutral
Neutral
Posted 15 April 2009 - 02:39 PM
You will probably need 2D resolution.
