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gradient to separate 1kDa proteins


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3 replies to this topic

#1 alessandro

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Posted 10 April 2009 - 06:40 AM

Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...

#2 mdfenko

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Posted 13 April 2009 - 07:25 AM

it depends on the actual mw of the proteins.

1kDa difference is more significant in a small protein than a large one.
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#3 gfischer

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Posted 15 April 2009 - 11:37 AM

Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...


You may not be able to. Westerns aren't necesarily exact, you usually need to give a band about a 5kDa margin of error.
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#4 mikew

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Posted 15 April 2009 - 02:39 PM

You will probably need 2D resolution.




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