gradient to separate 1kDa proteins
Posted 10 April 2009 - 06:40 AM
Posted 13 April 2009 - 07:25 AM
1kDa difference is more significant in a small protein than a large one.
genius does what it must
i do what i get paid to do
Posted 15 April 2009 - 11:37 AM
Hi all, i would know at what % should can i make a gel gradient to separate 2 proteins that differs each other only for 1kDa...
You may not be able to. Westerns aren't necesarily exact, you usually need to give a band about a 5kDa margin of error.
then heaven will be yours, before you meet your end