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Which statistics to use- HELP!!!


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#1 LostintheLab

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Posted 09 April 2009 - 08:38 PM

HELP!!! Sadistics....sorry Statistics is nightmare for me and I need help in choosing which test to carry out.

I have done some phenotypic cells counts, 15 fields with each sample and 3 phenotypic characteristics, I have each represented as a percentage of the total cell number.
What I want to check is whether there is a statistically significant change between the same phenotype when the cells are undifferentiated and then differentiated.
Please let it be an easy one! I only have access to excel at the moment. Something like a T-test?

a speedy reply would be great as I can add it to my talk for tomorrow.

Lost ;)
I knew it! I knew it! Well, not in the sense of having the slightest idea, but I knew there was something I didn't know.

#2 pcrman

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Posted 09 April 2009 - 10:21 PM

Because you are doing non-parametric stats, you can use chi-square 2x3 table to calculate. Here is a excel temple which allows you to do that as found here http://www.runet.edu...2X3template.xls

Attached File  Copy_of_chi_sq2X3template.xls   17KB   28 downloads

There is also an online tool allows you to calculate Fisher exact probability http://faculty.vassa.../fisher2x3.html

#3 LostintheLab

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Posted 10 April 2009 - 12:59 AM

Thanks!! you're a life saver....
Chi squared- yes! I know this one and I understand it, which is more important.

Seriously, I don't really understand most statistics, I have missed out on this in my previous education somehow.


A much happier Lost ;)
I knew it! I knew it! Well, not in the sense of having the slightest idea, but I knew there was something I didn't know.

#4 pcrman

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Excellent

Posted 10 April 2009 - 08:38 PM

You are welcome.




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