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northern blot: strange results


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#1 Jane2009

Jane2009

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Posted 09 April 2009 - 01:32 PM

Hello everyone! I met a strange problem. In my northern blotting result, on the X-ray film, there are a bright band of each lane(sense membrane), while, in the same size, dark band of each lane appear in my anti-sense membrane in desired size. That is, dark bands in anti-sense membrane are bright bands in sense membrane. I don't know why? How to explain this appearance. What are the reasons?
Thanks!
If you have any suggestion or idea, Please contact with me! My email: zhangjiexjtu_09@hotmail.com




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