lactamase, on Apr 14 2009, 05:35 AM, said:
pito, on Apr 10 2009, 06:08 PM, said:
lactamase, on Apr 10 2009, 06:42 AM, said:
Oh, Sorry for that....
Actually, I am trying to growth it, but I also want to keep a stock in -80oC.....but I don't know whether it is possible or not....
It is a freezedried culture you bought?
If so ,then why not store it as a it is?
You can also email the company for more details, but I think it should not be a problem to store it in liquid nitrogen if you know how to. Most bacteria can be stored like that without problems, however some are harder to store or require other methods.
The bacteria is in lyophilized form, and the amount is very small. So, I am wondering whether I should use this tiny amount lyophilized bacteria to prepare forzen stock or growth it. Anyway, I aliquot it into 2.
Actually, the story is that. I ordered this bacteria, but it took about 6 months and ATCC replied me that this bacteria is difficult to grow.
Therefore, I am really afraid that I do something wrong, and I have to wait for another 6 months..........
Just out of curiosity, how "big" is your sample then? x gram?
And be sure to keep a bit in the freezedried form, just in case something goes wrong.
And why do you doubt between growing it and store it in -80°C? You do not need it at the moment for your work then? (you do not need to grow it at the moment?)
Anyway, a little hint: some websites contain lists with a species kept in liquid nitrogen at their institute... you should search a bit on those websites or ask the institute if you cant find it. So you will find out whether they have your bacteria in liquid nitrogen or not... so you will know if its possible to store it like that or not.
Anyway, I think it should not be a problem anyway. Just try it as phage434 said and be sure not to corrupt your sample.
Good luck with it.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.