antibody for immunocytochemistry
Posted 08 April 2009 - 12:45 PM
Posted 08 April 2009 - 02:14 PM
Posted 13 April 2009 - 06:32 PM
As to the species, if you're using an Ab that was raised against an antigen of a different species you can always do a protein alignment (using ensembl or similar) and see how high the homology is between the species the antigen was taken from and the species of your tissue / cells. I worked in zebrafish and no one makes anti-fish abs, but there are plenty of other abs out there that do just fine.
Also, do a literature search for your antibody and see if it's used for immunofluorescence in the literature at all. Chances are you won't find it because they probably would have referenced it on the data sheet, but you never know.
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!
If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
Posted 21 April 2009 - 02:56 AM
and unfolded (western after denaturation)