I'm writing up a lab report and I was wondering if anybody had a link to a good explanation to the theory behind a phenol chloroform extraction of DNA?
Thanks
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#2
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Hmmm, I think it's working
Posted 08 April 2009 - 04:49 PM
Not a link, but try finding a copy of Sambrook, Fritsch and Maniatis, Molecular Cloning: A Laboratory Manual.
#3
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Posted 08 April 2009 - 05:52 PM
Different solubilities. Nucleic acids are more soluble in water, and thus predominate in the aqueous phase, whereas proteins prefer an organic solvent, and thus separate to the phenolic phase.
