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Phenol chloroform extraction of DNA


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#1 Nilafhiosagam

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Posted 08 April 2009 - 05:07 AM

I'm writing up a lab report and I was wondering if anybody had a link to a good explanation to the theory behind a phenol chloroform extraction of DNA?

Thanks

#2 bob1

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Posted 08 April 2009 - 04:49 PM

Not a link, but try finding a copy of Sambrook, Fritsch and Maniatis, Molecular Cloning: A Laboratory Manual.

#3 HomeBrew

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Posted 08 April 2009 - 05:52 PM

Different solubilities. Nucleic acids are more soluble in water, and thus predominate in the aqueous phase, whereas proteins prefer an organic solvent, and thus separate to the phenolic phase.

#4 Nilafhiosagam

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Posted 09 April 2009 - 07:51 AM

Thank you for the replies.




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