10 replies to this topic

[lactamase]

Hi all

I just purchased a new bacteria, but it come as a sealed glass vial........how should I open that vial ? just hit it hard and then open it ?

is there some trick to do that ?

Thx

[Dr Teeth]

lactamase, on Apr 8 2009, 04:53 AM, said:

Hi all

I just purchased a new bacteria, but it come as a sealed glass vial........how should I open that vial ? just hit it hard and then open it ?

is there some trick to do that ?

Thx

Usually, in these type of containers, the top part of the vial snaps off easily.

[hobglobin]

I remember that some chemicals/samples/sera are delivered in glass vials without lid. You have to slit slightly the neck of the vial with a special saw and then break it open.
Don't know if you meant this kind of vial or if bacteria are delivered in them

[klinmed]

lactamase, on Apr 8 2009, 10:53 AM, said:

Hi all

I just purchased a new bacteria, but it come as a sealed glass vial........how should I open that vial ? just hit it hard and then open it ?

is there some trick to do that ?

Thx

Go to the ATCC website (www.atcc.org). They have a document entitled "Instructions for Rehydrating Freeze-Dried Cultures". This organisation is the expert in such matters.

Hope this helps.

[Doki]

lactamase, on Apr 8 2009, 05:53 PM, said:

Hi all

I just purchased a new bacteria, but it come as a sealed glass vial........how should I open that vial ? just hit it hard and then open it ?

is there some trick to do that ?

Thx

hobglobin, on Apr 9 2009, 01:04 AM, said:

I remember that some chemicals/samples/sera are delivered in glass vials without lid. You have to slit slightly the neck of the vial with a special saw and then break it open.
Don't know if you meant this kind of vial or if bacteria are delivered in them

If it is this type of vial then the white mark does mean that the top can be easily snapped off but like Hobglobin suggested, it might be better to use a small saw as snapping can go wrong and U might spill some of your samples.

[phage434]

The ampules shown in the picture are intended to be scored with a triangular file, then snapped off.

I'm guessing that the original poster has an ATCC sealed glass ampule.  These are easy to open by heated the sealed end of the tube in a bunsen burner for a minute or so (get it quite hot).  Then spray it with 70% ethanol.  It will crack, but not shatter.  use a paper towel to cover it and break the vial open, removing the interior vial containing the sample.  This interior vial is stoppered with a cotton plug, which can be removed with sterile tweezers.

[lactamase]

phage434, on Apr 9 2009, 10:46 AM, said:

The ampules shown in the picture are intended to be scored with a triangular file, then snapped off.

I'm guessing that the original poster has an ATCC sealed glass ampule.  These are easy to open by heated the sealed end of the tube in a bunsen burner for a minute or so (get it quite hot).  Then spray it with 70% ethanol.  It will crack, but not shatter.  use a paper towel to cover it and break the vial open, removing the interior vial containing the sample.  This interior vial is stoppered with a cotton plug, which can be removed with sterile tweezers.

Thx everyone and Mr phage, and I just want to add the photo of the vial that I reveived from ATCC

Attached Thumbnails

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Edited by lactamase, 08 April 2009 - 07:29 PM.

[miraclestrain]

Hi there,

            From the photo displayed it looks like the special media which comes in a vial when using an API.

To open the vial all you need is one plastic cap with a depression on the it. You have to cover the vial with the cap just like capping a normal ink pen.

After capping use your thumb finger to push the head of the vial (i.e. on the cap)

The bulb glass will break leaving you to access the solution inside.

Remember once you open it you cannot recap the vial. Transfer the contents into the bacterial growth medium

Cheers

[Majlinda]

[b]Hi!

I have made some test of identification microorganisms present in beer. The result was bacteria bacilli Gram Positive, Katalase positive, and the shape of this colonies is viscose. through the IDFlowChart i saw that they belong to Brevi bacterium, microbacterium and Cyronobacterium. but i want to know Haw much is the damage of these bacteria present in the product?

[pito]

Majlinda, on Mar 18 2010, 11:00 AM, said:

[b]Hi!

I have made some test of identification microorganisms present in beer. The result was bacteria bacilli Gram Positive, Katalase positive, and the shape of this colonies is viscose. through the IDFlowChart i saw that they belong to Brevi bacterium, microbacterium and Cyronobacterium. but i want to know Haw much is the damage of these bacteria present in the product?

Wrong place for this topic.

you should make a new topic in the microbiology forum.

[Majlinda]

pito, on Mar 18 2010, 04:45 PM, said:

Majlinda, on Mar 18 2010, 11:00 AM, said:

[b]Hi!

I have made some test of identification microorganisms present in beer. The result was bacteria bacilli Gram Positive, Katalase positive, and the shape of this colonies is viscose. through the IDFlowChart i saw that they belong to Brevi bacterium, microbacterium and Cyronobacterium. but i want to know Haw much is the damage of these bacteria present in the product?

Wrong place for this topic.

you should make a new topic in the microbiology forum.

thx for the suggestions. i am new in a forum and i maybe can make some mistake