Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -


  • Please log in to reply
2 replies to this topic

#1 march



  • Active Members
  • Pip
  • 14 posts

Posted 07 April 2009 - 02:24 AM

Hi All,

how to look for the reporter gene such as GFP in specific cell type in a transgenic mice.Do we need to cross the Tg mice with the GFP reporter mice and are there any GFP reporter mice commercially available.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,504 posts

Posted 07 April 2009 - 05:14 PM

If you mean a cell-type specific expression of a mutant gene labelled with GFP, then just crossing the mutant mice with GFP won't work because the expression of the GFP could and most likely will be independent of the expression of the mutant gene. You would need to create a mutant construct that expresses GFP and then insert it into an embryo and get the mice from there.

#3 kifayath



  • Active Members
  • Pip
  • 8 posts

Posted 08 April 2009 - 06:04 AM

If you have already a Tg mouse with fused GFP to your gene of interest, by default you should see the expression of GFP under a flourescence microscope. For specific cell types, say hippocampal neurons, if the GFP expresses, you can see the flourescence. in some cases, though the GFP correctly fused, but u might not get flourescence, in this case just use anti-GFP antibody to see the expression. Hope i am correct.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.