ELISA Extraction Buffer for Plants?
Posted 06 April 2009 - 01:32 PM
I have previously used SDS based buffers to do Western Blots, but SDS isn't compatible with ELISA because it denatures antibodies. I have had extremely limited success trying to use PBST (phosphate buffered saline with Tween-20).
Any advice would be helpful.
Posted 09 April 2009 - 11:45 AM
Posted 17 April 2009 - 07:20 AM
After you do the extraction could you not change buffers by dialysis?
I could potentially do that. However, that is an entire extra step. I would only do that if it is the only way. Additionally, extra steps like that make ELISA less quantitative because there are extra steps with extra opportunity for human error.
Does ANYONE work on plants here?
Posted 22 April 2009 - 08:30 AM
Posted 28 May 2009 - 12:49 AM
I don't work with arabidopsis but I work on some crop plants like Groundnut, Cotton, Pigeon pea, Sunflower where I use PBST or TBST @ pH-7.6 or 50mM Tris at pH-8.0 as ELISA extraction buffers. Check with these buffers it may help you.
All the best