Does anyone have a recipe for a "gentle" IgG elution buffer? I'm doing a Protein G magnetic bead purification of cell culture supernatant and I need an elution buffer that won't denature the protein G or antibody. I would buy a commercially available buffer, but I really want to get this done today! Thanks
0
1 reply to this topic
#2
Carlton H
-
- Active Members
-
- 95 posts
Enthusiast
0
Neutral
Neutral
Posted 06 April 2009 - 10:13 AM
You could try making a 0.1M glycine-HCl solution and keep the pH high (> 3). Also, be sure to add an alkaline buffer immediately afterward to neutralize the eluted fraction and minimize denaturation (Tris works).
NextAdvance
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!
If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!
If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
