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"Gentle" IgG elution

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#1 trexbob



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Posted 06 April 2009 - 07:20 AM

Does anyone have a recipe for a "gentle" IgG elution buffer? I'm doing a Protein G magnetic bead purification of cell culture supernatant and I need an elution buffer that won't denature the protein G or antibody. I would buy a commercially available buffer, but I really want to get this done today! Thanks

#2 Carlton H

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Posted 06 April 2009 - 10:13 AM

You could try making a 0.1M glycine-HCl solution and keep the pH high (> 3). Also, be sure to add an alkaline buffer immediately afterward to neutralize the eluted fraction and minimize denaturation (Tris works).
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