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RNA isolation form plants


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4 replies to this topic

#1 Makosad

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Posted 06 April 2009 - 05:49 AM

Hi
I want to isolate RNA from oranges. any prefereable method or kit avaiable which I can use to isolate RNA without contaminating DNA present or both seperately.
Thanks

#2 bob1

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Posted 06 April 2009 - 04:41 PM

Try Trizol or Tri-reagent, you will need to do something about the oil from the skin if you are using the fruit.

#3 molgen

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Posted 07 April 2009 - 05:54 AM

Witch part of the orange are you working on?
If it's the exocarp or mesocarp I'd use a protocol with CTAB preferably containing PVP.
If I remember correctly in oranges they are called flavedo and albedo.
CTAB especially with PVP extract RNA very well in flavonoid and polysaccharide rich tissues.
Try these situations:
Chang S., Puryear J. and Cairney J. (1993). A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Report 11:113-116.
or this site
DNA fingerprinting in plants By Kurt Weising, Hilde Nybom, Kirsten Wolff has 2 protocols

Edited by molgen, 07 April 2009 - 06:05 AM.


#4 molgen

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Posted 07 April 2009 - 06:07 AM

I googled "orange RNA extraction" and got this:
An Effective Protocol for the Isolation of RNA From the Pulp of Ripening Citrus Fruits

#5 Makosad

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Posted 08 April 2009 - 04:48 AM

I googled "orange RNA extraction" and got this:
An Effective Protocol for the Isolation of RNA From the Pulp of Ripening Citrus Fruits

I am going to try the extraction from flavedo.
Thanks.




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