I need a protocol for biotinylation of 5' end of RNA. all I found was the protocols of labeling with 32P.
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#1
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Posted 05 April 2009 - 02:24 AM
hi all,
I need a protocol for biotinylation of 5' end of RNA. all I found was the protocols of labeling with 32P.
I need a protocol for biotinylation of 5' end of RNA. all I found was the protocols of labeling with 32P.
#2
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Posted 05 April 2009 - 04:44 PM
Rimal,
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
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If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
#3
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Posted 06 April 2009 - 03:32 PM
Carlton H, on Apr 5 2009, 05:44 PM, said:
Rimal,
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
Can you explain more?
