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5' end RNA biotin labeling


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#1 rimal

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Posted 05 April 2009 - 02:24 AM

hi all,

I need a protocol for biotinylation of 5' end of RNA. all I found was the protocols of labeling with 32P. :P

#2 Carlton H

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Posted 05 April 2009 - 04:44 PM

Rimal,

This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.

-Carlton
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#3 rimal

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Posted 06 April 2009 - 03:32 PM

Rimal,

This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.

-Carlton



Can you explain more?




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