Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

4% w/v paraformaldehyde


  • Please log in to reply
2 replies to this topic

#1 Biologist

Biologist

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 03 April 2009 - 02:18 PM

which company's 4% w/v paraformaldehyde you'd buy?
I bought one bottle from BD, but the purity of paraformaldehyde is only 90-93%, is this good enough for in situ hybridyzation?

Thanks.

#2 noakes84

noakes84

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 05 April 2009 - 12:55 PM

which company's 4% w/v paraformaldehyde you'd buy?
I bought one bottle from BD, but the purity of paraformaldehyde is only 90-93%, is this good enough for in situ hybridyzation?

Thanks.


I think we use Sigma PFA, and use it for in-situ hybridisation in zebrafish with no problems, check what purity this is on their website, its the bog-standard one if they do lots of different types!

Chris

#3 Carlton H

Carlton H

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 95 posts
0
Neutral

Posted 06 April 2009 - 10:40 AM

PFA is one of those things that, in most applications, you can use with a fairly large margin of error. 90% pure should be fine.

If you're new to in-situs, the #1 piece of advice I can give is to make sure you fully prewarm all your solutions when you're doing the washes at ~66*C. In-situs are finicky and if you don't follow the protocol well they often won't work. ... Feel free to get in touch if you have any questions.

-Carlton
NextAdvance
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!

If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.