2 replies to this topic

[scoob00]

Hi everyone,

I have a quick question, just to make sure what I am doing is correct...

I need to determine the concentration of my protein in order to use it as a standard. It is pure. So, I have worked out E, by adding the number of W, Y and disulphide bonds in the mature protein.

I then take this value, with the determined molecular weight (MALDI) and use the 'Other Protein, E & MW' function on the nanodrop to determine the concentration.

Is this good enough? And correct?

Thanks,
L

[scoob00]

Hello everyone,

I've noticed I have had no replies to this post - can anyone tell me why? Have I either a) not given enough information, or nobody uses the nanodrop or c) something else?!

Cheers!
Scoob

[sudhakar mutyala]

scoob00, on May 19 2009, 03:55 AM, said:

Hello everyone,

I've noticed I have had no replies to this post - can anyone tell me why? Have I either a) not given enough information, or nobody uses the nanodrop or c) something else?!

Cheers!
Scoob

Go for simple methods of protein estimation like bradford or lowry or Spectophotometric