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I am looking for a smart way to design a positive control for FACS measures of activation markers (like HLA-DR, CD25, CD69, CD38) across cell types from whole blood.
It's hard to find literature on what could be a stimulator or stimulator combination for such an approach.
I am currently thinking of PHA (or SEA or B)for TC, PMA (and ionomycin?) or SEB for NK and LPS (or PMA) for monocytes..
Does anybody have a good recommendation for a suitable combination and concentrations for these activators?
Thinking of using whole blood:medium (1:1), 4-6h at 37 and then stain and FACS.
Any ideas?
Gre
oh and I forgot: how long should I incubate at 37C to see a difference?
Edited by Gre, 02 April 2009 - 08:10 PM.
