Im looking for a protocol for electroporation of HEK293 cells and cant seem to find a particularly good one.
What do you suspend the cells in and at what density? How much DNA do you add? How do you keep the cells nice after electroporating?
When i transform JM109 e.coli i have to pellet and wash with ice cold distilled water 4 times then resuspend in 2 volumes of water before electroporating them to remove salts. I dont see anything like this for HEK?
Edited by yer_maw, 02 April 2009 - 08:27 AM.