Cell Wall Lysis For Dummies
Posted 01 April 2009 - 12:53 PM
My goal is to extract the contents of "spirulina" (I think chlorella) cells for use as a food colorant. I'd like to maintain the bluish emerald colors that come from the different pigments, but in my brief experimentation, boiling the sample in water appears to harm the blue pigments, and the solution becomes yellow/green. Exposing the sample to a low pH solution (H2O and citric acid) extracts a small amount of sky-blue color, and nothing else. A 20% alcohol solution seems to be retaining good color, but I think it may just be keeping the cells in suspension. I'd like to have the majority of the proteins fall out of suspension.
I've read about a freeze-thaw method, but it seems to always mention conducting the freeze-thaw cycles in some kind of buffer (pH buffer?) and with detergents.
Are there any food safe methods of breaking the cell walls that do not require highly specialized equipment or chemicals?
The spirulina is in dried powder form, and was purchased as a dietary supplement.
Thanks a lot.
Posted 02 April 2009 - 05:14 AM
or even a sonicator if you can get your hands on one.
Posted 02 April 2009 - 08:08 AM
Posted 02 April 2009 - 08:11 AM
to remove excess liquid place in a water bath or oven to evaporate
Posted 02 April 2009 - 01:23 PM