Posted 01 April 2009 - 03:34 AM
I have used TMRE to measure mitochondrial membrane potential for SW480 cancer cells treated with an agent which induces apoptosis after 24 hrs of exposure. I aimed to measure the mitochondrial membrane potential after 12 hrs of exposure, where I collected the cells by trypsinization, and then loaded them with 100nM TMRE for 10 minutes at 37 oC then kept them on ice and immediately analysed them within 1 hr. I aquired 20,000 cells and did not use a gate. FSC threshold was set to 52. As demonstrated on the histograms in the attached JPEG file, following drug treatment, there is an increase in population (I) and a reduction in population (II), as compared to control, while I was expecting the opposite. Actually I can not explain this reproducible result and I greatly appreciate your responses.
thank you very much.
Posted 08 June 2009 - 06:35 AM
Did you manage to figure out the reason? I have a similar problem at the moment and would be grateful for advice.
Posted 08 June 2009 - 07:05 AM
Posted 08 June 2009 - 07:10 AM
Posted 09 June 2009 - 01:53 PM
Posted 10 June 2009 - 09:52 AM