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Live/dead bacteria count


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#1 SuMi

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Posted 01 April 2009 - 02:34 AM

Hi,

Does anyone know how to do a live/dead bacteria count using microscopy? I am currently doing bacterial counts using a microscope but I can't separate between live and dead cells. I know there are lots of fluorescent live/dead stains but I don't want to use a fluorescent stain. Is there anything similar to trypan blue which is used for live/dead staining of mammalian cells that can be used for bacteria?

Thanks for any help or suggestions.

#2 molgen

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Posted 07 April 2009 - 06:30 AM

You could probably read the optical density at 600nm and then plate.
It's a labor intensive and time consuming way.




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