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Gel Electrophoresis Interpretation


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#1 ston

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Posted 31 March 2009 - 07:14 PM

In the digest:

pBluescript (one HindIII restriction site)
HindIII

The gel shows 4 bands, I cannot think of why there would be that many. The only thing I can think of is that pBluescript is circlular and the bands are due to different levels of supercoiling, but I feel like if that was the case then the gel would show more of a smear than distinct bands.

Any thoughts?

#2 phage434

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Posted 31 March 2009 - 07:18 PM

Have you compared this with uncut plasmid? Incomplete digestion would leave that present and may have (as you suggest) several bands. Perhaps you are trying to cut too much DNA?

#3 swanny

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Posted 31 March 2009 - 10:04 PM

Any chance of a gel image?
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#4 T C

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Posted 31 March 2009 - 10:58 PM

Yeah its uncut DNA




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