In the digest:
pBluescript (one HindIII restriction site)
HindIII
The gel shows 4 bands, I cannot think of why there would be that many. The only thing I can think of is that pBluescript is circlular and the bands are due to different levels of supercoiling, but I feel like if that was the case then the gel would show more of a smear than distinct bands.
Any thoughts?
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#2
phage434
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Posted 31 March 2009 - 07:18 PM
Have you compared this with uncut plasmid? Incomplete digestion would leave that present and may have (as you suggest) several bands. Perhaps you are trying to cut too much DNA?
#3
swanny
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Posted 31 March 2009 - 10:04 PM
Any chance of a gel image?
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#4
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Posted 31 March 2009 - 10:58 PM
Yeah its uncut DNA
