18 replies to this topic

[pito]

In my former department, we placed our wet autoclaved tip boxes into a heated drying cabinet. It took a while but the boxes come out dry the next morning

I know, we have that system too, but it is costly.

[HeatherH]

On a similar note, my DEPC-H2O protocol requires a sterilization time of 15min per 1L of water. Why is the increase in sterilization time necessary?

[nicolas]

Just a general notice:

Why are you autoclaving tips? To clean them?

In my lab, like thousand other labs, we have only one autoclave used for tips and cell garbages. Sometime it happen that bags containing garbages open, and then everything in the autoclave is contaminated. And I am wonder for next cycles too.

Personal opinion, I prefer close safely tips bags and don' t autoclave tips.

[kiikyo]

You can autoclave them together.

Our lab has a very old autoclave, similar to yours, which only has an on/off switch and timer setting. We use 20min for solids only and 25min for liquids (or solids + liquids).

Tips must be sterile when working with bacteria, for example, which is why they are autoclaved -- to avoid any potential contamination.