primers fpr sequencing
Posted 30 March 2009 - 02:54 PM
I had have inserts in some vector like pMW172, pcDNA3 and pcDNA3 FLAG, and I want before starting gene expression to check the inserts by sending them for sequencing so I need a help to find forward and reverse primers for these vector/ inserts.
Thank you in advance
Posted 30 March 2009 - 04:09 PM
Posted 30 March 2009 - 05:01 PM
Posted 31 March 2009 - 06:08 AM
If I remember correctly, pMW172's back bone is pET. If I'm right, then it to has a T7 sequencing primer.
Posted 03 April 2009 - 03:13 AM