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lyophilized protein now looks clear


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#1 scoob00

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Posted 30 March 2009 - 03:10 AM

Hi everyone,

About a year ago I did a big protein extraction/purification and stored aliquots of my freeze dried protein. They used to look white and powdery as usual.
However, I have just gone to get a fresh one, and they no longer took white and powdery - more like a little lump of clear goop sticking to the side of the tube. What has happened? Will it still be ok do you think?

Any help greatly appreciated....

:P

#2 mdfenko

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Posted 30 March 2009 - 11:13 AM

the sample seems to have hydrated.

it may still be okay if the protein can survive freezing.
talent does what it can
genius does what it must
i do what i get paid to do

#3 bob1

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Posted 30 March 2009 - 04:18 PM

Use better tubes next time, most of the tubes without little silicone gaskets in them will slowly leak, especially at low temperatures.

#4 scoob00

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Posted 02 April 2009 - 05:24 AM

Thanks very much. I have used an aliquot today and so far so good. Tubes were just standard 05.ml eppendorfs, so I'll consider an alternative for next time.

thanks again!




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