3 replies to this topic

[scoob00]

Hi everyone,

About a year ago I did a big protein extraction/purification and stored aliquots of my freeze dried protein. They used to look white and powdery as usual.
However, I have just gone to get a fresh one, and they no longer took white and powdery - more like a little lump of clear goop sticking to the side of the tube. What has happened? Will it still be ok do you think?

Any help greatly appreciated....

[mdfenko]

the sample seems to have hydrated.

it may still be okay if the protein can survive freezing.

[bob1]

Use better tubes next time, most of the tubes without little silicone gaskets in them will slowly leak, especially at low temperatures.

[scoob00]

Thanks very much. I have used an aliquot today and so far so good. Tubes were just standard 05.ml eppendorfs, so I'll consider an alternative for next time.

thanks again!