Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

PCR with cell source


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 13 October 2001 - 09:00 PM

Does anyone know of a protocol for PCR where I can start with whole cells and lyse them right there in the PCR tubes as I'm doing it?

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 15 October 2001 - 09:00 PM

I use this method routinely for colony screening. If you are using bacterial cells.... Make up your PCR cocktail as normal and aliquot into as many tubes as needed. With a toothpick or tip pick one bacterial colony and swirl tip into cocktail. Don't worry you will have enough DNA. As the temperature raises to 95 degrees the cells will lyse. You can also save the tip in a culture tube during the PCR, that way when you know which are your positive clones you can start O/N cultures for minipreps.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.