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shRNA from SIGMA


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5 replies to this topic

#1 dvddecarvalho

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Posted 29 March 2009 - 08:47 AM

Hey guys,
Anybody here has already used the shRNA vectors from the shRNA! MISSION TRC sold by SIGMA-ALDRICH?
I wanna knockdown some genes and I a friend told me about those vectors.

Thanks

#2 pcrman

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Posted 29 March 2009 - 09:16 AM

What specific do you want to know about shRNA from Sigma? I believe all vectors are verified for gene knockdown.

#3 dvddecarvalho

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Posted 29 March 2009 - 09:30 AM

I just wanna know if someone here has good or bad experience with those vectors.
If people are used to buy the lentiviral particules or plasmid DNA to make their own viral particules.

#4 pcrman

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Posted 31 March 2009 - 08:34 PM

I believe their vector system should work fine and should have been validated by numerous labs.

#5 dvddecarvalho

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Posted 01 April 2009 - 04:19 AM

Ok,
Thank you

Dvd

#6 Functional Screens

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Posted 03 April 2009 - 08:00 PM

TRC (MIT Broad Institute) has generated 45,000-75,000 lenti-shRNA vector for both human and mouse. Some of them work fine, some of them do not work well. It may be because of the algorithm. However, all shRNAs ARE NOT VALIDATED by TRC.




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