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fungi in antibody working solution


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2 replies to this topic

#1 Curtis

Curtis

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Posted 29 March 2009 - 01:33 AM

hi all,

I usually keep my working solution of primary antibody in 1%BSA-PBST at 4 degrees for immunofluorescence experiments. Today I was about to use the antibody (which is 1:100 dilution) that I saw it has fungi in it.

to be honest I don't know if it's really fungi or BSA crystals but it didn't look good for IF incubation anyway.

I made another solution though but I want to know if I can re-use this antibody in case I filter it with a 0.22 um or 0.45 um filters?

#2 bob1

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Posted 29 March 2009 - 03:38 PM

I wouldn't use it, who knows what the fungus is secreting into the solution, and what it has used up. If you want to preserve the antibody solutions a little better, you can add 0.01% sodium azide.

#3 Curtis

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Posted 30 March 2009 - 12:41 AM

thank you bob1

you are right, we don't know what the fungus is secreting.

I will have to throw away the solution although it was a very concentrated antibody (60 ug in 6 ml). next time I'll add sodium azide to not let fungi grow.




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