Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Playing for keeps


  • Please log in to reply
3 replies to this topic

#1 hanming86

hanming86

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 156 posts
2
Neutral

Posted 28 March 2009 - 11:19 PM

Hi,

I did SDS PAGE on cell lysate ( lysed with 1X sample buffer) yesterday. I still have some left . After boiling the sample , i used some for SDS PAGE and kept the remaining in 4C.

My question is , can i still use the sample for furhter SDS PAGE? or do i need to prepare fresh one?

it seems to me that it's not neccesary because if it's needed , it woudl have been recommended in the protein ladder marker product that we bought all the time.

i woudl like to further confirm this so ur suggestion would really help on this end.

thanks.
Lab + Coffee + Music = Bliss

#2 Sciurus

Sciurus

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 28 March 2009 - 11:47 PM

I don't see a reason why not to use the rest of your lysate - if stored properly... ;)

#3 T C

T C

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 277 posts
0
Neutral

Posted 29 March 2009 - 12:56 AM

Hey,

Use it, it works.

TC

#4 rkay447

rkay447

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 180 posts
20
Excellent

Posted 29 March 2009 - 03:32 PM

This sample will work fine on a new gel. I routinely do experiments large enough to get at least two gels worth of sample. This way, if there is a problem in the gel or transfer, I can re-run samples.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.