Playing for keeps
Posted 28 March 2009 - 11:19 PM
I did SDS PAGE on cell lysate ( lysed with 1X sample buffer) yesterday. I still have some left . After boiling the sample , i used some for SDS PAGE and kept the remaining in 4C.
My question is , can i still use the sample for furhter SDS PAGE? or do i need to prepare fresh one?
it seems to me that it's not neccesary because if it's needed , it woudl have been recommended in the protein ladder marker product that we bought all the time.
i woudl like to further confirm this so ur suggestion would really help on this end.
Posted 28 March 2009 - 11:47 PM
Posted 29 March 2009 - 03:32 PM