i've troubles with amino acid starvation on C6 rat glioma cells.
the first "simple" trouble concerns dissolving some insoluble amino acids (i.e. cystine) to prepare stock solutions: can somebody show a good practise without strongly acting on pH of th solutions?
second: can someone tell me if cell culture synchronization effectively has some impact on starvation?
thanks for every kind of answer...
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amino acid starvation
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