Posted 28 March 2009 - 09:41 AM
Does anyone know a decent equipment that would be able to quantify plasmid DNA better than the Nanodrop? I don't want to waste too much sample and was hoping that the Nanodrop might do an OK job. Turned out that when I use the Nanodrop it always tells me that my samples are of low concentration, but when I run them on gel they show up has having really bright bands! So I now have to linearize my plasmids, run them on a gel and roughly quantify them using the High Mass Ladder (Invitrogen), which takes so much time! Does anyone have a better idea? The Invitrogen Qubit also sucks at quantifying plasmid DNA too.