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Quantifying plasmid DNA


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#1 ruiN

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Posted 28 March 2009 - 09:41 AM

Does anyone know a decent equipment that would be able to quantify plasmid DNA better than the Nanodrop? I don't want to waste too much sample and was hoping that the Nanodrop might do an OK job. Turned out that when I use the Nanodrop it always tells me that my samples are of low concentration, but when I run them on gel they show up has having really bright bands! So I now have to linearize my plasmids, run them on a gel and roughly quantify them using the High Mass Ladder (Invitrogen), which takes so much time! Does anyone have a better idea? The Invitrogen Qubit also sucks at quantifying plasmid DNA too.

Thanks!

#2 mastermi

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Posted 28 March 2009 - 10:26 AM

Probably your Nanodrop has to be recalibrated. Or do you have a special buffer to keep your plasmids in?

For me it always works perfectly. For DNA aswell as for RNA.

#3 hanming86

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Posted 29 March 2009 - 03:30 AM

U might want to mix the solution properly prior to doing nanodrop. taking it from just the surface sometimes gave low reading. from my experience it' s better to figner vortex and then pipet few times b4 taking the sample for measurement.
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