19 replies to this topic

[elzattara]

Hello,

I did some tests on a bacteria and i don't have a Bergey's manual to find out what bacteria I have. I already know it's NOT neisseria.Could someone help me to find out what gram-negative coc this ? (sorry for my bad english)

1. morphological properties

shape: coc
Position : disorder
Gram : G-
KOH: visceus + G-
Aminopeptidase: +  G-

2. culture characteristics

view on SBA
Diameter: 2-3 mm
Shape: round
Hoogte: flat
Rand: intact
Oppervlak: shiny
Lijnenting op SBA: even at the growth line
Steekenting in SBG: growth
Steekenting in SBG: no run (still intact...)
Motility: +-, not really agile
Citraat: Koser-citraat: -   Koser is more important then simmons => citraat -
Simmons-citraat: +
Malonaat: -


3. Biochemical characteristics

sugar conversion  gas acid

Glucose + +
Lactose + +
Sucrose + +




Kligler (KIA) + + + -
TSI + + + -


Methylrood /Voges Proskauer
MR: +
VP: -
ONPG: +
Amylase: -
Gelatinase Clarcke: -
O/F-test Glucose Lactose Sucrose

+/+ +/+ +/+

Urease: +
Fenylalanine: -
Indool: -
Decarbosylase-testen:
Basis: geel
Lysine: paars
Arginine: geel
Ornithine: geel
Lecithenase: -
Katalase: +-
Oxidase:
Papier: -
Disc/strip: -
Nitraatreductie: +
Vorming van:
NO2: +
N2: -
Coagulase:
Slidetest: -
Tubetest: -
DNase: -

Edited by elzattara, 28 March 2009 - 03:49 AM.

[pito]

could it be Veillonella?
are you from Holland, Belgium or Suriname since you speak dutch?

[elzattara]

Ja , ik ben van België
Volgende week heb ik waarschijnlijk een Bergey's manual ter beschikking en ga ik daar even in rondneuzen. Alvast bedankt voor de tip, ik zal er eerst eens speciaal op letten als mijn testjes overeen lijken te komen met Veillonella.  

edit: ik veronderstel dat er zo geen online versie bestaat van de Bergey's manual? (ik vind toch niet direct iets )

Edited by elzattara, 28 March 2009 - 11:48 AM.

[pito]

elzattara, on Mar 28 2009, 09:33 PM, said:

Ja , ik ben van België
Volgende week heb ik waarschijnlijk een Bergey's manual ter beschikking en ga ik daar even in rondneuzen. Alvast bedankt voor de tip, ik zal er eerst eens speciaal op letten als mijn testjes overeen lijken te komen met Veillonella.  

edit: ik veronderstel dat er zo geen online versie bestaat van de Bergey's manual? (ik vind toch niet direct iets )

Indeed there is no Bergeys online manual.
There is however a flow chart that is online and you can get it here:
flowcharts, this is also how I got the Veillonella, I did not have time really to check Bergey's and I do not have acces to it at the moment.

It does seem strange that you are able to do so many labtests, but have no acces to Bergeys.
Are you working at a university or?

PS. I know dutch and understand you, but the others here dont

[elzattara]

Yes i'm still going to highschool. I need to find it untill next week. And i know it's a gramnegative coc just from thursday. So I don't have alot of time and I never used Bergey's manual, so i put it here. I thought i could get some hints for bacteria, so I can directly look in Bergey's manual if they match.

[elzattara]

update: i did my gramtest again and it looks like it are NO cocs. So it looks that i have a facultatively anaerobic gramn-negative rod.
I searched in my papers and it looks like I have Klebsiella ozaenae. Only the urease test should be negative (i have positif) and dulcitol should be negative and mine is positif. Is this negligible?

grtz

[pito]

elzattara, on Mar 31 2009, 05:53 PM, said:

update: i did my gramtest again and it looks like it are NO cocs. So it looks that i have a facultatively anaerobic gramn-negative rod.
I searched in my papers and it looks like I have Klebsiella ozaenae. Only the urease test should be negative (i have positif) and dulcitol should be negative and mine is positif. Is this negligible?

grtz

Klebsiella species do produce urease if I am not mistaken, so your test should be positive like you said.

Maybe you need to do a H2S test, Klebsiella ozaenae produces H2S

[elzattara]

hmm my manual says its H2S negative, just like my K. ozaenae. Everything matches exept the dulcitol test (is positive, should be negative).

[pito]

elzattara, on Apr 1 2009, 08:22 PM, said:

hmm my manual says its H2S negative, just like my K. ozaenae. Everything matches exept the dulcitol test (is positive, should be negative).

Yes I was wrong, K. species does NOT produce H2S.
I ment to say: test it, and it if does not produce H2S its K. ozaenae.


However Klebsiella is urease positive, wich you have so thats ok.

are you sure the dulcitol test needs to be negative?

Check  the attachement. I havent had time to read it in depth, but it might help you.

Attached Files

•  38_3217.pdf   347.24K   957 downloads

[GeorgeWolff]

Think klebsiella may be your bug.

note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.

[pito]

GeorgeWolff, on Apr 3 2009, 03:06 AM, said:

Think klebsiella may be your bug.

note - all the enterics produce H2S - it's a matter of amount that is used to differentiate among them.

Are you sure about that? In literature they refer to it as no production.

[hanming86]

another feature of klebsiella is they are quite slimy. most klebsiella i have seen are like that.

[GeorgeWolff]

Yes pito - the literature for differentiation refers to a technique that doesn't detect lesser amounts.  Put lead acetate paper in a tube of Klebsiella and it will demonstrate presence of H2S.

[pito]

GeorgeWolff, on Apr 14 2009, 12:22 AM, said:

Yes pito - the literature for differentiation refers to a technique that doesn't detect lesser amounts.  Put lead acetate paper in a tube of Klebsiella and it will demonstrate presence of H2S.

Ah I see, interesting, I have indeed also found in some papers that they refer to "no production" at all when they really mean: a very small amount of H2S production. This is really misleading.

But you are refering to Klebsiella now as an organism that produces H2S, but do you also mean that ALL enterics (Enterobacteriaceae) produce H2S ?
You could be right.

Anyway, interesting topic , I have looked around a bit and it doenst seem easy to make a correct point on the matter (see attachments)

Attached Files

•  206.pdf   451.55K   238 downloads
•  H2S.pdf   503.75K   64 downloads

Edited by pito, 14 April 2009 - 07:46 AM.

[aimikins]

a negative stain with India Ink should show you the presence/absence of a capsule?