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Viscous RNA samples


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#1 Pancho

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Posted 27 March 2009 - 06:09 AM

Hello everyone,

For the first time in my life I extracted RNA from plant tissue using TRI reagent (SIGMA). Quick and easy. However, when I took my samples out of the freezer to take an aliquot to evaluate the integrity on a gel, I noticed that the samples became viscous, hence difficult to pipette. I don't know what this happened or if it good or bad. Any ideas?

Another thing that has me really worried. Last week I ran a MOPS gel to verify RNA integrity and it looked fine. This week I ran a second gel of new extractions along with the old ones (last week's gel). When I took the picture of my gel I was very worried since all my samples appeared to be degraded. I really hope that the sole reason this happened is because I used DEPC water to prepare my first gel and ultrapure water to prepare the second. Is it possible that the RNA degraded for no apparent reason?

#2 Curtis

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Posted 27 March 2009 - 08:57 AM

most probably you have genomic DNA in your sample. I have the same problem recently. my friends told me to buy DNase I and add to my RNA samples from now on.....I am also using TRI




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