Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Polysaccharides co-precipitatingwith protein in ammonium sulfate


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
0
Neutral

Posted 12 November 2001 - 10:00 PM

We purify proteins from fleshy fruits and sometimes something we suspect is polysaccharides precipitates as a yelly material when performing ammonium sulfate protein precipitation. We'd like to get rid of that yelly material beforesubmission to ammonium sulfate precipitation.May someone help me? Thanx

#2 seiko

seiko

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 22 September 2003 - 07:23 AM

Hi there,
I have a same problem to precipitate proteins.
I do TCA precipitation and cannot remove polysaccharide.
To see clear bands in SDS-PAGE, I need to remove polysaccharide.
Proteins are precipitated from supernatant of bacterial culture.
Does anybody know how to remove extracellular polysaccharide?
Thanks,




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.