4 replies to this topic

[heejun]

Resently, I want to isolate LPS from E.coli by hot phenol/water method which was described by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. I have an ergent need to get it. Is there anybody who can help me to get this protocol? Thank you.  

[pito]

heejun, on Mar 27 2009, 08:18 AM, said:

Resently, I want to isolate LPS from E.coli by hot phenol/water method which was described by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. I have an ergent need to get it. Is there anybody who can help me to get this protocol? Thank you.  

There was a similar topic like yours here a few years ago ( http://www.protocol-...sts/13636.html), however due to the servercrash this is lost,but maybe you should email Charlie Ellis: c.ellis@imperial.ac.uk on the matter. He has experience with the protocol you need, he also has the protocol, so he might be able to help you.

If he doenst answer you, you could try and email: Dr. Y. Veeranagouda at veeresha_y@rediffmail.com, he has also the protocol, I think.

I hope this helps.

Edited by pito, 27 March 2009 - 01:12 PM.

[heejun]

pito, on Mar 28 2009, 05:05 AM, said:

heejun, on Mar 27 2009, 08:18 AM, said:

Resently, I want to isolate LPS from E.coli by hot phenol/water method which was described by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. I have an ergent need to get it. Is there anybody who can help me to get this protocol? Thank you.  

There was a similar topic like yours here a few years ago ( http://www.protocol-...sts/13636.html), however due to the servercrash this is lost,but maybe you should email Charlie Ellis: c.ellis@imperial.ac.uk on the matter. He has experience with the protocol you need, he also has the protocol, so he might be able to help you.

If he doenst answer you, you could try and email: Dr. Y. Veeranagouda at veeresha_y@rediffmail.com, he has also the protocol, I think.

I hope this helps.

Thank you very much. It's really very nice of you. And I will try to email the prof.

[pito]

the link doesnt seem to work anymore and I can not edit the message thus herehere is the corect link.

(http://www.protocol-...osts/13636.html)

If it is possible heejun, could you then upload the protocol here or maybe send it to me?

thanks.

[pito]

Anyone who reads this topic or needs info on the subject, be sure check the files attached.

Attached Files

•  lps_protocol_extractionkit.pdf   159.65K   686 downloads
•  role_of_phosphoglucomutase_of_stenotrophomonas_maltophilia_in_lipopolysaccharide_biosynthesis_virulence_and_antibiotic_resistance.pdf   178.77K   191 downloads
•  For_Core_LPS.pdf   21.48K   157 downloads
•  Ag_LPS_staining.pdf   5.9K   137 downloads

Edited by pito, 25 April 2009 - 07:53 AM.