Posted 26 March 2009 - 07:43 AM
Hi, im trying to clone two genes coding for a similar protein from a parasite. Theres supposed to be no introns in my gene. My problem is that the primers are exactly complementary to other regions of the genome (which is extremely weird in my opinion). Thus its quite hard to clone the genes I want, I just have tried and tried and it didnt produce. I finally got some inserts to ligate today and did a digestion analysis...my gene is supposed to be around 2kb and I got 1 kb long products. What should I do to clone these genes? I cant just make different primers, as I need the gene from start to finish with added restriction sites and proteolysis site at the end.