I want to check for teh mythlation status of my gene in a panel of cell lines that show no expression of my gene of interest. I have never done this and all help would be appreciated.
1) I check for methylation by first treating with 5 azacytidine
2) to confirm by bisulphite treatment and MSP PCR.
Does anyone have teh protocol for 5 azacytidine treatment, I have no experience on this. Also, where can I purchase this?
No replies to this topic