Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Effect of sonication on membrane anchored proteins


  • Please log in to reply
1 reply to this topic

#1 victor.m

victor.m

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 25 March 2009 - 09:47 AM

Hi all.
The protein of my interest should associate with plasma membrane. There are different options how to disrupt cells for isolation membranes. Now I am interested in sonication.
I would like to ask you:
Do you have any experience with isolation of membrane (e.g., microsomes, plasma membrane) proteins, when the cells are disrupted by sonication?
Would sonication (for example, something like 3 x 20 sec., on ice, 50% duty cycle) not lead to total disruption of membranes and thus releasing membrane-anchored proteins from membrane (protein would not be any more in membrane)?
Does sonication disrupt all cell compartments, i.e. nuclear, mitochondrial proteins are also released during sonication?

Thanks for your comments.
vic

#2 genz

genz

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 02 April 2009 - 07:35 PM

Hi there,

Sonication would eventually disrupt all cell compartment, nucleus, mitochondia etc...
But I do not know if peripheral protein would still stick to disrupted membrane fragment.
You can also treat your protein lysate with high sale (1M NaCl) or extreme pH (100 mM Na2CO3 pH 11.5) to release peripheral membrane proteins.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.